DNA-Based Identification of Forensically Important Lucilia (Diptera: Calliphoridae) in the Continental United States

• NCJ Number: 243730
• Journal: Journal of Forensic Sciences Volume: 58 Issue: 1 Dated: January 2013 Pages: 73-78
• Author(s): Ronald W. DeBry, Ph.D.; Alicia Timm, Ph.D.; Evan S. Wong, B.S.; Trevor Stamper, Ph.D.; Clifford Cookman, B.S.; Gregory A. Dahlem, Ph.D.
• Date Published: October 2013
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Technical Assistance)
• Language: English
• Country: United States of America

Annotation

This study used phylogenetic analysis as the basis for DNA-based species identification.

Abstract

Correct species identification is critical when dipteran larvae are used for inference of the postmortem interval. To facilitate DNA-based identification of forensically important flies of the genus Lucilia in the continental United States, the researchers developed a vouchered reference collection and DNA sequence database. A total of 122 specimens were collected for 9 of the 10 species of Lucilia reported to occur in the continental United States. Using the polymerase chain reaction and DNA sequencing, data were obtained for an 1100-bp region of the mitochondrial gene encoding cytochrome oxidase I (COI). Researchers consider a species suitable for DNA-based identification if it is exclusively monophyletic in greater than 95 percent of bootstrap pseudoreplicate phylogenetic analyses. Seven of the nine species meet that criterion. Two species (Lucilia coeruleiviridis and Lucilia mexicana) share COI sequence and cannot be distinguished using the reference database. Researchers conclude that DNA-based identification is likely to be successful for the other seven species. Abstract published by arrangement with John Wiley & Sons.