In this paper, the authors describe a novel method using resistive pulse sensing to selectively detect short target DNAs and report the use of the variable pressure method for target DNA detection.
Despite the plethora of DNA sensor platforms available, a portable, sensitive, selective and economic sensor able to rival current fluorescence-based techniques would find use in many applications. In this research, probe oligonucleotide-grafted particles are used to detect target DNA in solution through a resistive pulse nanopore detection technique. Using carbodiimide chemistry, functionalised probe DNA strands are attached to carboxylated dextran-based magnetic particles. Subsequent incubation with complementary target DNA yields a change in surface properties as the two DNA strands hybridize. Particle-by-particle analysis with resistive pulse sensing is performed to detect these changes. A variable pressure method allows identification of changes in the surface charge of particles. As proof-of-principle, the authors demonstrate that target hybridization is selectively detected at micromolar concentrations (nanomoles of target) using resistive pulse sensing, confirmed by fluorescence and phase analysis light scattering as complementary techniques. The advantages, feasibility, and limitations of using resistive pulse sensing for sample analysis are discussed. (Publisher Abstract Provided)
Downloads
Similar Publications
- A Self-assessment Tool for Helping Identify Police Burnout Among Investigators of Child Sexual Abuse Material
- Postmortem CT Scans Supplement and Replace Full Autopsies
- Improving Outcomes for Child and Youth Victims of Commercial Sexual Exploitation: An Evaluability Assessment of the Love 146 Survivor Care Programs