DNA re-identification is used for a broad suite of applications, ranging from cell line authentication to forensics; however, current re-identification schemes suffer from high latency and limited access. In addressing this issue, MinION sketching requires as few as 3 minutes of sequencing and 60-300 random SNPs to re-identify a sample, enabling near real-time applications of DNA reidentification. The method used in the current project capitalizes on the rapidly growing availability of genomic reference data for cell lines, tissues in biobanks, and individuals. This empowers the application of MinION sketching in research and clinical settings for periodic cell line and tissue authentication. Importantly, this method enables considerably faster and more robust cell line authentication relative to current practices and could help to minimize the amount of irreproducible research caused by mix-ups and contamination in human cell and tissue cultures. (publisher abstract modified)
Downloads
Similar Publications
- Dyed Hair and Swimming Pools: The Influence of Chlorinated and Nonchlorinated Agitated Water on Surface-Enhanced Raman Spectroscopic Analysis of Artificial Dyes on Hair
- Extraction of Ignitable Liquid Residues by Dynamic Capillary Headspace Sampling and Comparison to the Carbon Strip Method
- Identification of Blunt Force Traumatic Fractures in Burned Bone