Achiral and Chiral Quantification of Methamphetamine and Amphetamine in Human Urine by Semi-micro Column High-Performance Liquid Chromatography and Fluorescence Detection

• NCJ Number: 183946
• Journal: Journal of Forensic Sciences Volume: 45 Issue: 3 Dated: May 2000 Pages: 708-714
• Author(s): Osama Y. Al-Dirbashi M.Sc.; Mitsuhiro Wada Ph.D.; Naotaka Kuroda Ph.D.; Masakatsu Takahashi Ph.D.; Kenichiro Nakashima Ph.D.
• Date Published: May 2000
• Length: 7 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America
• Note: *This document is currently unavailable from NCJRS.

Annotation

This article describes procedures for determining the presence of methamphetamines and amphetamines in human urine.

Abstract

The article describes miniaturized achiral and chiral high-performance liquid chromatographic procedures for determining the presence of methamphetamines and amphetamines in human urine. After a simple pretreatment of human urine (i.e., the urine was acidified and dried-up at room temperature) and fluorescence derivatization, the derivatives were isocratically separated. Wide-ranged calibration curves were obtained with detection limits for the achiral and chiral analyses in the atto and femtomol levels, respectively. Satisfactory within- and between-day reproducibility data were obtained with both methods, with the highest relative standard deviation being 9.6 percent. The concentrations of methamphetamine and amphetamine in human urine as determined by the two methods were well correlated (r = 0.994). Because of their excellent sensitivity, selectivity and reproducibility, and the significant reduction in analysis time, solvents and samples, the two methods are possible simple-to-perform alternatives for routine urinalysis in forensic and clinical application. Figures, tables, references