Applicability of the SNPforID 52-plex Panel for Human Identification and Ancestry Evaluation in a Brazilian Population Sample by Next-Generation Sequencing

• NCJ Number: 254233
• Journal: Forensic Science International-Genetics Volume: 40 Dated: May 2019 Pages: 201-209
• Author(s): Guilherme do Valle-Silva; Flavia D. Nunes de Souza; Leticia Marcorin; Alison L. Eburneo Pereira; Thassia M. Telles Carratto; Guilherme Debortoli; Maria L. Guimaraes de Oliveira; Nadia C. de Agular Fracasso; Edilene Santos de Andrade; Eduardo A. Donadi; Heather L. Norton; Esteban J. Parra; Aguinaldo L. Simoes; Erick C. Castelli; Celso T. Mendes-Junior
• Date Published: May 2019
• Length: 9 pages
• Grant Number(s): 2013-DN-BX-K011
• Sponsoring Agency: National Institute of Justice (NIJ)
• Publication Format: Article
• Publication Type: Research (Applied/Empirical); Report (Study/Research); Report (Grant Sponsored); Program/Project Description
• Language: English
• Country: United States of America

Annotation

This study evaluated the informativeness of the SNPforID 52-plex system in a Brazilian population sample (n - 340).

Abstract

SNP analysis is of paramount importance in forensic genetics. The development of new technologies in next-generation sequencing allowed processing a large number of markers in various samples simultaneously. Although SNPs are less informative than STRs, they present lower mutation rates and perform better when using degraded samples. Some SNP systems were developed for forensic usage, such as the SNPforID 52-plex, from the SNPforID Consortium, containing 52 bi-allelic SNPs for human identification. DNA libraries were prepared using a customized HaloPlex Target Enrichment System kit (Agilent Technologies, Inc.) and sequenced in the MiSeq Personal Sequencer platform (Illumina Inc.). The methodology presented in this article enabled the analysis of 51 out of 52 SNPforID markers. Allele frequencies and forensic parameters were estimated, revealing high informativeness: the combined match probability and power of exclusion were 6.4810 -21 and 0.9997, respectively. Population admixture analysis indicates high European contribution (more than 70 percent) and low Amerindian contribution (less than 10 percent) in our population, while individual admixture analyses were consistent with the majority of individuals presenting high European contribution. This study demonstrates that the 52-plex kit is suitable for forensic cases in a Brazilian population, presenting results comparable with those obtained using a 16 STR panel. (publisher abstract modified)