Bone Marrow and Bone as a Source for Postmortem RNA

• NCJ Number: 234662
• Journal: Journal of Forensic Sciences Volume: 56 Issue: 3 Dated: May 2011 Pages: 720-725
• Author(s): Nienki L. van Doorn, M.Sc.; Andrew S. Wilson, Ph.D.; Eske Willerslev, D.Sc.; M.Thomas P. Gilbert, Ph.D.
• Date Published: May 2011
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

This study examined RNA degradation after death.

Abstract

The susceptibility of RNA to enzymatic degradation has been considered as a tool to estimate time-since-death in forensic samples, and it has previously been demonstrated that the choice of tissue is an important factor. In this study the authors have extracted RNA from decaying bone and bone marrow under the hypothesis that the delayed onset of putrefaction may render them a useful source in this context. In a preliminary study, total RNA was extracted from bone and bone marrow that had been sampled from six skeletally mature rabbits at time points between zero and 31 days after death. The levels of three specific RNA transcripts could be quantified using real-time polymerase chain reaction. Bioanalyzer results show rRNA bands in bone marrow samples up to 21 days postmortem. The authors hereby propose bone marrow as a potential source for postmortem RNA in forensic studies. (Published Abstract)