NCJ Number
183900
Journal
Journal of Forensic Sciences Volume: 44 Issue: 6 Dated: November 1999 Pages: 1258-1260
Date Published
November 1999
Length
3 pages
Annotation
This study analyzed Allele and genotype frequencies for the five PCR-based loci in 157 unrelated Turkish individuals randomly selected from criminal cases.
Abstract
The DNA was extracted from fresh blood leucocytes, bloodstains, tissues, and single hairs following the published methods. The five PCR-based loci included LDLR, GYPA, HBGG, D7S8, and Gc. The extracted DNA was amplified and typed for the PM loci by using AmpliType PM PCR Amplification and Typing Kit according to the manufacturer's protocol. Amplification was carried out in a Perkin Elmer DNA Thermal Cycler 480. The frequency of each of the five loci was calculated from the numbers of each genotype in the sample. Unbiased estimates of expected heterozygosity were computed as described by Edwards, et al. The hypothesis that the genotype frequency distribution for each of the five loci conform to the HWE was tested by using the chi-square and exact tests. The results of the chi-square and exact tests showed that the genotype distribution at the LDLR, GYPA, D7S8, and Gc loci did not significantly differ from the Hardy-Weinberg Expectation (HWE); however, the genotype distribution at the HBGG locus did not conform to HWE. Moreover, the genotype frequencies calculated in this study were compared with the published genotype frequencies of U.S. African-American and U.S. Caucasian populations. The Turkish population was significantly different at the HBGG locus from the U.S. Caucasian population; however, there were highly significant differences at the LDLR, HBGG, and Gc loci between the Turkish and African-American populations. 3 tables and 11 references