Determination of Phenmetrazine in Urine by Gas Chromatography-Mass Spectrometry After Liquid-Liquid Extraction and Derivatization with Perfluorooctanoyl Chloride

• NCJ Number: 172021
• Journal: Journal of Forensic Sciences Volume: 42 Issue: 5 Dated: (September 1997) Pages: 937-941
• Author(s): A Dasgupta; C E Mahle
• Date Published: 1997
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America
• Note: DCC

Annotation

This article describes a process for determining the presence in urine of phenmetrazine.

Abstract

Phenmetrazine is a central nervous system stimulant and is currently used as an anorectic agent. The drug is abused and reported to cause death from overdose. This study describes a liquid-liquid extraction protocol for phenmetrazine from urine using l-chlorobutane and subsequent derivatization using perfluorooctanoyl chloride for gas chromatography-mass spectrometric confirmation. Quantitation of urinary phenmetrazine can be easily achieved by using N-propylamphetamine as an internal standard. The article describes: cross reactivity of phenmetrazine with the EMIT assay for urinary amphetamines; chromatographic properties of derivatized phenmetrazine; mass spectral characteristics of perfluorooctanoyl derivative of phenmetrazine; precision linearity and detection limit; and application of the assay. Figures, table, references