NCJ Number
173726
Date Published
1998
Length
19 pages
Annotation
This report describes the development and evaluation of two types of DNA testing.
Abstract
Both Restriction Fragment Length Polymorphism (RFLP) and Short Tandem Repeat Polymerase Chain Reaction (STR PCR) DNA testing were developed and implemented at the Sedgwick County Regional Forensic Science Center in Kansas. The major activities of this project included development and validation of both types of DNA testing, laboratory modifications, and the establishment of a CODIS Local Network. The STR PCR DNA instrumentation includes three Perkin Elmer 2400 thermal cyclers with a temperature verification system, Hitachi FMBIO and a remote DNA analysis station, and four electrophoresis workstations. The FMBIO was chosen because of prospects of high throughput, flexibility for use with multiple analysts and different systems, ease of interpretation, simple procedures, and low maintenance. The ventilation of the PCR lab was specifically designed to prevent contamination of PCR amplified products by exhausting the lab from 150 to 450 CFM. The CODIS local network includes a server, four client work stations, one scanning workstation and a LaserJet printer which reside on a token-ring Novel NetWare network. The report includes proceedings from the Eighth International Symposium on Human Identification (1997). Tables, figures