Development and Application of Immunoassay for Paraquat: Radioimmunoassay

• NCJ Number: 118061
• Journal: Journal of Forensic Sciences Volume: 34 Issue: 3 Dated: (May 1989) Pages: 547-552
• Author(s): M Nagao; T Takatori; K Terazawa; B Wu; C Wakasugi; M Masui; H Ikeda
• Date Published: 1989
• Length: 6 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

A sensitive radioimmunoassay for paraquat is reported.

Abstract

Anti-paraquat antisera were produced by repeated immunization in rabbits with 1-methyl,1'-hexanoic acid-4,4'-bipyridinium (MHBP) coupled to bovine serum albumin (BSA). Less than 0.5 ng of paraquat dichloride was detectable by this assay system. These antisera were strongly cross-reactive with the bipyridyl ring and methyl group in either the 1- or 1'-position of paraquat. The determination of paraquat in tissues of paraquat-poisoned cadavers was also carried out. (Author abstract)