NCJ Number
236671
Journal
Forensic Science International: Genetics Volume: 5 Issue: 5 Dated: November 2011 Pages: 415-421
Date Published
November 2011
Length
7 pages
Annotation
This study presented the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes for identity and kinship testing with degraded DNA.
Abstract
This study presents the development and characterization of two X chromosomal short tandem repeat (STR) multiplexes utilizing reduced-size amplicons (less than 200 base pairs) for identity and kinship testing with degraded DNA. Aproximately 1,360 samples across 4 U.S. population groups were typed for 15 X chromosomal STR markers: DXS6789, DXS7130, DXS9902, GATA31E08, DXS7424, GATA165B12, DXS101, DXS6795, GATA172D05, DXS10147, DXS8378, DXS7132, DXS6803, HPRTB, and DXS7423. A high degree of polymorphism was observed for each marker and both multiplexes were sensitive down to 200pg of pristine DNA. The two proposed multiplexes are suitable for forensic use, and show potential for improved analysis of compromised bone samples. (Published Abstract)