Effects of Solvent Displacement on Sensitivity and Specificity of Monoclonal Antibodies for ABO Blood Grouping of Forensic Specimens with an Absorption-Elution Test

• NCJ Number: 181274
• Journal: Legal Medicine Volume: 1 Issue: 2 Dated: April 1999 Pages: 68-75
• Author(s): Tetsuya Kobayashi; Makoto Yokota; Tomoaki Mitani; Atsushi Akane
• Date Published: 1999
• Length: 8 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: Japan

Annotation

Using commercially available monoclonal antibodies (Bioclone, Neo Kokusai, Monoclonal Wako, Gamma Clone, and Seraclone), ABO blood grouping of forensic specimens such as bloodstains, salivary stains, seminal stains, nails, hair, and cerebral dura mater was performed with an absorption-elution test.

Abstract

Salivary stains, seminal stains, and nails were not typed correctly using antibodies other than Bioclone reagents, while precise grouping of bloodstains was performed using most antibodies. When hair and dura mater were tested, all antibodies induced weak or non-specific haemagglutination; hence, correct grouping was not achieved. When antibody solvents were displaced with 5-20 percent bovine serum albumin in saline, human serum of the group AB donor or serum of chicken, sheep, or bovine, titers of reagents increased 2-8 times. Hair and dura mater were typed using Bioclone reagents after solvent displacement with human AB or sheep serum, whereas displacement with the other solvents enhanced non-specific reactions. 20 references and 8 tables