NCJ Number
245527
Journal
Forensic Science International: Genetics Volume: 9 Dated: March 2014 Pages: 12-19
Date Published
March 2014
Length
8 pages
Annotation
This study established a novel short tandem repeat (STR) multiplex using 13 tetra-nucleotide STRs and the amelogenin marker for the forensic identification of pigs.
Abstract
This study established a 14-plex polymerase chain reaction (PCR) amplification system that included 13 tetra-nucleotide repeat short tandem repeat (STR) and the amelogenin markers. The development of these markers has been in accordance with the recommendations of the ISFG Commission on the use of non-human DNA. The genotypes and allele frequency of 341 samples from 11 pig breeds in Taiwan were established. From these data, the parameters of genetic variation (Na, Ne, Ho, He, F-statistics, and PIC), Pm and PE were calculated for each STR locus and each breed. Based on the 341 samples in this study, the CPm and CPE trio of the 13 loci were 1.31E-11 and 0.9996, respectively. The system established could be used as a tool in the studies for pig individualization, parentage testing, breed assessment, phylogenetic study, and forensic applications. The forensic relevance of this project stems from the fact that pig (Sus scrofa) is one of the most important domesticated species and can often be in close contact with humans; e.g., recently a number of important cases occurred in Taiwan in which pig-related material was essential to an investigation. 3 tables, 4 figures, and 32 references