Exploiting Expressed Sequence Tag Databases for the Development and Characterization of Gene-Derived Simple Sequence Repeat Markers in the Opium Poppy (Papaver somniferum L.) for Forensic Applications

• NCJ Number: 236181
• Journal: Journal of Forensic Sciences Volume: 56 Issue: 5 Dated: September 2011 Pages: 1131-1135
• Author(s): Eun Jung Lee, Ph.D.; Gang Nam Jin, M.D.; Kyung Lyong Lee, Ph.D.; Myun Soo Han, Ph.D.; Yang Han Lee. Ph.D.; Moon Sik Yang, Ph.D.
• Date Published: September 2011
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

Simple sequence repeat (SSR) markers in the opium poppy (Papaver somniferum L.) were identified from an expressed sequence tag (EST) database comprised of 20,340 sequences.

Abstract

In total, 2780 SSR-containing sequences were identified. The most frequent microsatellite had an AT/TA motif (37 percent). Twenty-two opium poppy EST-SSR markers were presently developed and polymorphisms of six markers (psom 2, 4, 12, 13, 17, and 22) were utilized in 135 individuals under narcotic control investigation. An average of three alleles per locus (range: 2-5 alleles) with a mean heterozygosity of 0.167 was detected. Six loci identified 29 unique profiles in 135 individuals. The EST-SSR markers exhibited small degrees of genetic differentiation (fixation index = 0.727, p less than 0.001). Other variable markers will be needed to facilitate the forensic identification of the opium poppy for future cases. To determine the potential for cross-species amplification, six markers were tested in five Papaver genera species and two Eschscholzia genera. The psom 4 and psom 17 primer pair was transferable. This is the first study to report SSR markers of the opium poppy. (Published Abstract)