NCJ Number
153528
Journal
Journal of the Forensic Science Society Volume: 34 Issue: 4 Dated: (October-December 1994) Pages: 231-235
Date Published
1994
Length
5 pages
Annotation
A simple and efficient method for extracting human genomic DNA from microsamples of blood is described that uses sodium perchlorate, chloroform, polymerized silica gel, and a dumbbell- shaped tube instead of proteinase K and phenol.
Abstract
The entire process takes less than 2 hours, and high molecular weight DNA in high yield and purity has been obtained from a few microliters of human blood. DNA prepared in this way can be easily digested with restriction endonucleases and has been employed for DNA profiling and polymerase chain reaction. The method offers several advantages; it is suitable for the specialized use of forensic requirements and offers a significant reduction in time, both for the operator and for the redissolution of DNA. Further, the method enables pure, high molecular weight DNA to be extracted in high yield, and it is especially suitable for forensic DNA profiling and polymerase chain reaction. Disadvantages of using the method are also noted. 15 references, 2 tables, and 2 figures