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Flow cytometry dataset for cells collected from touched surfaces

NCJ Number
308366
Journal
F1000Research Volume: 5 Issue: 390 Dated: 2016
Author(s)
Ye Jin Kwon; Cristina E. Stanciu; M. Katherine Philpott; Christopher J. Ehrhardt
Date Published
2016
Annotation

This paper describes a dataset comprising the results from antibody hybridization surveys using Human Leukocyte Antigen (HLA) and Cytokeratin (CK) probes, as well as surveys of optical properties of deposited cells.

Abstract

This paper describes a dataset comprising the results from antibody hybridization surveys using Human Leukocyte Antigen (HLA) and Cytokeratin (CK) probes, as well as surveys of optical properties of deposited cells, including forward scatter (FSC), side scatter (SSC), and fluorescence emissions in the Allophycocyanin (APC) channel. All analyses were performed on “touch” samples deposited by several different contributors on multiple days to assess inter- and intra-contributor variability. ‘Touch’ or trace cell mixtures submitted as evidence are a significant problem for forensic laboratories as they can render resulting genetic profiles difficult or even impossible to interpret. Optical signatures that distinguish epidermal cell populations from different contributors could facilitate the physical separation of mixture components prior to genetic analysis, and potentially the downstream production of single source profiles and/or simplified mixtures.   (Published Abstract Provided)