NCJ Number
241109
Journal
Forensic Science International: Genetics Volume: 7 Issue: 1 Dated: January 2013 Pages: 143-150
Date Published
January 2013
Length
8 pages
Annotation
In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges.
Abstract
In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges. Messenger RNA (mRNA) profile clearly reveals tissue-specific gene expression patterns that many attempts have been made to use RNA for forensic tissue identification. To systematically investigate the body-fluid-specific expression of mRNAs and find novel mRNA markers for forensic body fluid identification, the authors performed DNA microarray experiment with 24 Korean body fluid samples. Shannon entropy and Q-values were calculated for each gene, and 137 body-fluid-specific candidate genes were selected. By applying more stringent criteria, the authors further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR. As a result, the authors suggest a novel combination of four body-fluid-specific mRNA makers: PPBP for blood, FDCSP for saliva, MSMB for semen and MSLN for vaginal secretion. Multiplex qRT-PCR assay was designed using the four mRNA markers and DNA/RNA co-extraction method was tested for forensic use. This study will provide a thorough examination of body-fluid-specifically expressed mRNAs, which will enlarge the possibility of practical use of RNA for forensic purpose. (Published Abstract)