NCJ Number
145765
Journal
Journal of Forensic Sciences Volume: 38 Issue: 4 Dated: (July 1993) Pages: 961-967
Date Published
1993
Length
8 pages
Annotation
This study demonstrated that amplification of human DNA sequences by Alu-polymerase chain reaction (PCR) could be accomplished in samples containing low concentrations of template in the presence of excess heterologous DNA sequences.
Abstract
Human fibroblasts derived from human foreskin were maintained in a humidified atmosphere. Rat liver epithelial cells, which carry individual human chromosomes, provided a source for complex DNA mixtures. Individual human chromosomes were introduced into a tumorigenic variant of rat liver epithelial cells via microcell-mediated chromosome transfer. Neomycin-resistant colonies were isolated using cloning rings and established as pure clones. DNA was isolated from cells maintained in tissue cultures using standard laboratory protocols. Bacterial DNA was prepared from Escherichia coli or Enterobacter cloacae. The Alu-PCR technique proved to be a rapid and unique method for identifying human DNA in complex biological samples. Alu-PCR amplification of human DNA isolated from tissue culture cells resulted in distinct bands of DNA when electrophoretically separated in agarose gels. Amplified fragments ranged in size from 0.5 to 3 kb. Study findings confirm the utility of the Alu-PCR technique in forensic science applications. 16 references, 1 table, and 3 figures