NCJ Number
172015
Journal
Journal of Forensic Sciences Volume: 42 Issue: 5 Dated: (September 1997) Pages: 897-906
Date Published
1997
Length
10 pages
Annotation
This article reports on an interlaboratory evaluation of Short Tandem Repeats using the CTT triplex and CTTv quadruplex.
Abstract
Typing results were compared for Short Tandem Repeats (STRs) at the GenBank loci HUMCSF1PO, HUMTPOX, HUMTH01, and HUMVWFA31. These STRs all have a nominal four basepair (bp) repeat. Seven different samples were distributed to 41 laboratories. The 34 laboratories that returned results used a wide variety of analytical systems. Comparable results were obtained for all samples at all loci when results were reported as an allelic name. Raw sizing results obtained from internal-lane sizing standards differed by nearly five bp at some loci. Many different factors contribute to this observed sizing variability, including choice of sizing standards and matrix composition. Although sizing results can be made more comparable by locus-specific offsets or calibration to a comprehensive set of alleles at each locus, samples typed to the allelic name can now be validly compared regardless of analytical method. Interlaboratory comparison of raw allelic size remains problematic. Tables, figures, references