MICRO PROCEDURE FOR FAST TYPING OF THE GENETIC VARIANTS OF PHOSPHOGLUCOMUTASE

• NCJ Number: 19935
• Journal: Journal of the Forensic Science Society Volume: 14 Issue: 2 Dated: (APRIL 1974) Pages: 151-157
• Author(s): B W GRUNBAUM
• Date Published: 1974
• Length: 7 pages
• Corporate Author: Forensic Science Soc
• Publication Format: Article
• Language: English
• Country: United Kingdom

Annotation

THIS PAPER DESCRIBES A PROCEDURE FOR THE FAST, SPECIFIC, AND SENSITIVE DETERMINATION OF ISOENZYMS OF THE VARIOUS GENETIC TYPES OF PHOSPHOGLUCOMUTASE USING SUBMICROLITER VOLUMES OF SPECIMEN.

Abstract

CELLULOSE ACETATE IS USED AS THE SUPPORTING MEDIUM FOR ELECTROPHORESIS. A MULTIPLE SAMPLE APPLICATOR PLACES UP TO EIGHT SAMPLES ON THE MEMBRANCE IN ONE OPERATION. FOLLOWING ELECTROPHORESIS, THE CELLULOSE ACETATE MEMBRANE IS INVERTED ONTO A PRECAST GEL CONTAINING A REACTION MIXTURE WHICH YIELDS AN INSOLUBLE COLORED END PRODUCT ON INCUBATION AT 37 DEGREES C. THE WHOLE PROCEDURE, INCLUDING ELECTROPHORESIS AND STAINING, IS COMPLETE IN 30-80 MINUTES. THE RESOLUTION OF THE VARIOUS ISOENZYMES IS ADEQUATE AND PERMITS READY DISCRIMINATION OF THE COMMON GENETIC TYPES OF PHOSPHOBLUCOMUTASE, NAMELY TYPES 1, 2-1 AND 2. (AUTHOR ABSTRACT)