Multiplex PCR for 11 X Chromosome STR Markers and Population Data From a Brazilian Amazon Region

• NCJ Number: 222076
• Journal: Forensic Science International: Genetics Volume: 2 Issue: 2 Dated: March 2008 Pages: 154-158
• Author(s): Elzemar Martins Ribeiro Rodrigues; Fabio Pereira das Neves Leite; Mara Helena Hutz; Teresinha de Jesus Brabo Ferreira Palha; Andrea Kely Campos Ribeiro dos Santos; Sidney Emanuel Batista dos Santos
• Date Published: March 2008
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Technical)
• Language: English
• Country: United States of America

Annotation

This study describes a multiplex PCR system that allows the simultaneous analysis of 11 short tandem repeats (STRs) associated to the X chromosome for use in both paternity and more complex forensic investigations.

Abstract

The work described demonstrates the possibility of simultaneous genotyping of 11 highly polymorphic X-STRs in a single multiplex PCR reaction, which can be used both in paternity investigations and in more complex forensic cases. The analysis of X-STRs has gained increasing interest in forensic practice, especially for testing in complex kinship cases. Although many X-STRs have been validated for forensic use, little is known about the variation of these polymorphisms in different populations of the world. This work describes a new multiplex system that allows the simultaneous analysis of 11 X-STR markers, for use both in paternity determination and more complex forensic cases. Additionally, genotyping results are presented from a sample of 324 individuals from Belem, Para, a city located in the Brazilian Amazon. Tables, references