Novel Method for ABO Genotyping Using a DNA Chip

• NCJ Number: 233518
• Journal: Journal of Forensic Sciences Volume: 56 Issue: S1 Dated: January 2011 Pages: S183-S187
• Author(s): Ken Watanabe, Ph.D.; Hiroshi Ikegaya, M.D., Ph.D.; Koichi Hirayama, M.S.; Hisako Motani, D.D.S., Ph.D.; Hirotaro Iwase, M.D., Ph.D.; Hiroto Kaneko, M.D., Ph.D.; Hisayo Fukushima, Ph.D.; Tomoko Akutsu, Ph.D.; Koichi Sakurada, D.D.S., Ph.D.
• Date Published: January 2011
• Length: 5 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

ABO genotyping is often performed to identify the blood type of decomposed samples, which is difficult to be determined by a serological test. This study developed a simple method for ABO genotyping using a DNA chip.

Abstract

In this method, polymerase chain reaction-amplified and fluorescent-labeled fragments in the ABO gene and primate-specific D17Z1 were hybridized with DNA probes on a chip designed to detect single nucleotide polymorphisms (SNPs) in the ABO gene and part of the D17Z1 sequence. Using blood samples from 42 volunteers and 10 animal species, the authors investigated whether the chip could be used to detect SNPs in the ABO gene and the D17Z1 sequence. This method was then applied to various forensic samples, and it was confirmed that this method was suitable for the simultaneous analyses of ABO genotyping and species identification. This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations. (Published Abstract)