One Year Variability of Peak Heights, Heterozygous Balance and Inter-Locus Balance for the DNA Positive Control of AmpFSTR© Identifiler© STR Kit

• NCJ Number: 233921
• Journal: Forensic Science International: Genetics Volume: 5 Issue: 1 Dated: January 2011 Pages: 43-49
• Author(s): A. Debernardi; E. Suzanne; A. Formant; L. Pene; A.B. Dufour; J.R. Lobry
• Date Published: January 2011
• Length: 7 pages
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: Ireland

Annotation

This paper is an exploratory analysis of post-mortem data from a selection of positive control experiments.

Abstract

Multivariate analyses of 205 positive control experiments in an AmpFSTR© Identifiler© STR kit were used to analyze the factors affecting peak heights at 16 loci. Peak heights were found to be highly correlated between loci and there was evidence for a difference in sensitivity of the two genetic analyzers in the blue channel. Heterozygous balance response at 10 loci was found to behave as a random variable following a beta-distribution with typical median values of 90 percent, without locus or genetic analyzer effect. Inter-locus balance at 16 loci was influenced by the blue channel effect and a temporal switch of unexplained origin. The implications of these results for the choice of minimum threshold values in quality control are discussed. (Published Abstract) 1 table, 9 figures, and 27 references