NCJ Number
146937
Journal
Japanese Journal of Legal Medicine Volume: 47 Issue: 6 Dated: (December 1993) Pages: 493-498
Date Published
1993
Length
6 pages
Annotation
The DNA fingerprinting method was applied to resolve a paternity case; DNA samples were extracted from the mother, child (4-month-old fetus), and alleged father.
Abstract
DNA extracted from blood samples was digested with the restriction endonuclease Hinf I and HAE III, size separated by agarose gel electrophoresis, and hybridized with a multilocus minisatellite probe. DNA fingerprinting patterns in the child and alleged father indicated possible paternity. Following the extraction of DNA from the infant's cord blood, the quantity of DNA isolated was determined on a spectrophotometer at 260 nm. The quality of DNA was assessed by electrophoresis in agarose gels. It was found that high- molecular-weight DNA could be recovered in large quantities from both cord and adult blood. 19 references, 2 tables, and 2 figures