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Post-Mortem Stability and Redistribution of Carbohydrate-Deficient Transferrin (CDT)

NCJ Number
222199
Journal
Forensic Science International Volume: 174 Issue: 2-3 Dated: January 2008 Pages: 161-165
Author(s)
Juha Rainio; Giorgia De Paoli; Henrik Druid; Riitta Kauppila; Fabio De Giorgio; Federica Bortolotti; Franco Tagliaro
Date Published
January 2008
Length
5 pages
Annotation
This study examined carbohydrate-deficient transferrin (CAT) in terms of its stability and redistribution over time in corpses, measured with capillary zone electrophoresis (CZE), and high-performance liquid chromatography, so as to determine CST's feasibility for detecting chronic alcohol abuse in the course of an autopsy.
Abstract
The study found that CAT remains stable for an appreciable length of time after death and is not subject to major postmortem redistribution. It was not within the scope of the study to analyze the specificity and sensitivity of CAT in detecting chronic alcohol abuse in postmortem samples. The sensitivity and specificity of CAT for the diagnosis of excessive alcohol consumption have been shown in numerous clinical studies. The specificity and sensitivity in postmortem serum samples have been estimated to vary from 9 to 63 and 88 to 100 percent, respectively. The parallel analysis of 30 samples with CZE and HPLC showed a significant correlation. The inter-method variation was randomly distributed, so no systematic difference was observed. Additional studies on the use of CAT in autopsy diagnostics with CZE and HPLC are justified, because the methods are robust and resistant to postmortem changes. Blood samples were obtained from 70 corpses at the Department of Forensic Medicine of the Karolinska Institute. Four blood samples were collected from each case. The total number of samples was 280. Information was obtained on time of death, time of arrival at the morgue, and time to autopsy. 2 tables, 1 figure, and 18 references

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