Rapid Wire-Based Sampling Method for DNA Profiling

• NCJ Number: 240848
• Journal: Journal of Forensic Sciences Volume: 57 Issue: 2 Dated: March 2012 Pages: 472-477
• Author(s): Tong Chen, Ph.D.; David E.A. Catcheside, Ph.D.; Alice Stephenson, B.App.Sc.; Chris Hefford, B.Sc.; K. Paul Kirkbride, Ph.D.; Leigh A. Burgoyne, Ph.D.
• Date Published: March 2012
• Length: 6 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

This paper reports the results of a commission to develop a field deployable rapid short tandem repeat (STR)-based DNA profiling system.

Abstract

This paper reports the results of a commission to develop a field deployable rapid short tandem repeat (STR)-based DNA profiling system to enable discrimination between tissues derived from a small number of individuals. Speed was achieved by truncation of sample preparation and field deployability by use of an Agilent 2100 BioanalyserTM. Human blood and tissues were stabbed with heated stainless steel wire and the resulting sample dehydrated with isopropanol prior to direct addition to a PCR. Choice of a polymerase tolerant of tissue residues and cycles of amplification appropriate for the amount of template expected yielded useful profiles with a custom-designed quintuplex primer set suitable for use with the BioanalyserTM. Samples stored on wires remained amplifiable for months, allowing their transportation unrefrigerated from remote locations to a laboratory for analysis using AmpFlSTR Profiler Plus without further processing. The field system meets the requirements for discrimination of samples from small sets and retains access to full STR profiling when required. Abstract published by arrangement with John Wiley & Sons.