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Recovery of DNA From Latent Blood After Identification by Fluorescein

NCJ Number
208113
Journal
Journal of Forensic Identification Volume: 54 Issue: 6 Dated: November/December 2004 Pages: 660-667
Author(s)
Laurie A. Martin; Catherine F. Cahill
Editor(s)
Alan L. McRoberts
Date Published
November 2004
Length
8 pages
Annotation
This paper demonstrates the successful recovery of DNA from a blood sample treated with fluorescein.
Abstract
Any evidence found at a crime scene is vital to solving a crime. Blood found at a crime scene is one of those critical pieces of evidence. Fluorescein is a new method for latent blood detection, but it has not been tested for its impacts on DNA recovery. This study attempted to determine whether DNA could be recovered from latent blood samples that have been treated with fluorescein. The study treated denim substrates with blood then used fluorescein on the denim to identify the blood and extract the blood from the denim using Chelex 100 Resin. The analysis determined that DNA could be successfully recovered from the treated samples. So, fluorescein may be used at crime scenes to detect latent blood stains without destroying DNA evidence. This provides an important advance in evidence collection and analysis. Next, the parameters of sensitivity and chemical interactions need to be examined. References

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