NCJ Number
163582
Journal
Canadian Society of Forensic Science Journal Volume: 29 Issue: 1 Dated: (March 1996) Pages: 29-32
Date Published
1996
Length
4 pages
Annotation
This study found that polymerase chain reaction results may not always be obtained for forensic samples even though sufficient DNA appears to be present by slot blot, and the researchers determined that Taq polymerase inhibitors present in samples may have produced the results.
Abstract
DNA was extracted using standard organic methods and ethanol precipitation and then quantified by stained yield gels and slot blot techniques. Typical samples in the experiments were obtained from two sets of decalcified human bones recovered from fresh water after 3 to 5 years, blood in fabric, blood on metal, and the flap of a sealed envelope. Using a combination of sample dilution, Thiopropyl Sepharose beads, BSA, and additional Taq polymerase made it possible to overcome inhibition of the polymerase. Although this treatment was used with some success in the laboratory, the researchers caution that it does not work with every sample. 2 references and 1 figure