Sex Determination of Bloodstains by the Use of a Ribonucleic Acid (RNA) Probe

• NCJ Number: 123900
• Journal: Journal of Forensic Sciences Volume: 34 Issue: 5 Dated: (September 1989) Pages: 1078-1081
• Author(s): R Kobayashi; S Matsuzawa
• Date Published: 1989
• Length: 4 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

An asymmetric ribonucleic acid (RNA) probe, representing a Y-chromosome specific nucleotide sequence, was applied to the sex origin of bloodstains by using a nonisotopic Southern hybridization method.

Abstract

The RNA probe was in vitro transcribed with the recombinant of the Y-chromosome specific deoxyribonucleic acid (DNA) sequence and transcription-vector Bluescript SK M13+. The preparation of the RNA probe is not difficult, but it does require a subcloning procedure which is unnecessary in the preparation of the DNA probe. The results indicate the superiority of the RNA probe over the previously studied DNA probe. The RNA probe has a high hybridization efficiency which increases the sensitivity of the test and shortens the period of time necessary for obtaining the resulting stains. 2 figures, 7 references. (Author abstract modified)