SOLID PHASE EXTRACTION OF MORPHINE FROM WHOLE BLOOD BY MEANS OF BOND ELUT CERTIFY COLUMNS

• NCJ Number: 142994
• Journal: Journal of Forensic Sciences Volume: 38 Issue: 3 Dated: (May 1993) Pages: 668-676
• Author(s): X Chen; A L C Hommerson; P G M Zweipfenning; J Franke; C W Harmen-Boverhof; K Ensing; R A de Zeeuw
• Date Published: 1993
• Length: 9 pages
• Publication Format: Article
• Publication Type: Research (Applied/Empirical)
• Language: English
• Country: United States of America

Annotation

This study evaluated the use of Bond Elut Certify columns for the isolation of morphine from whole blood. In order to monitor possible losses and the elution profile of morphine, a small amount of tritiated analogue was added to the samples.

Abstract

Four sample pretreatment methods, three protein precipitation methods, and one sonication/dilution method, were tested. The sonication/dilution method produced the best results. The blood sample was applied onto the column at pH 3.3 after sonication and dilution with 0.1 M phosphate buffer (pH 3.3). Morphine retention was affected by the pH of the samples; the loss of morphine during sample application was minimized at pH 3.3. Interferences were removed by washing the column with the phosphate buffer, 0.01 M acetic acid, and methanol. Ammoniated methanol was used to elute morphine, with the result that more than 80 percent of 3H-morphine was recovered for concentrations of morphine ranging from 5 to 4000 ng/mL. 1 table, 5 figures, and 14 references