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Two-dimensional Thin-layer Chromatography of Ganja (Cannabis sativa L)

NCJ Number
119770
Journal
Bulletin on Narcotics Volume: 35 Issue: 1 Dated: (January-March 1983) Pages: 63-67
Author(s)
S N Tewari; J D Sharma
Date Published
1983
Length
5 pages
Annotation
The most widely found cannabis preparation is the ganja, the flowering tops of Cannabis sativa L.
Abstract
This paper describes an efficient and reliable two-dimensional thin layer chromatographic technique for the separation and identification of cannabinoids present in the resin of cannabis. A total of 47 different cannabinoids were successfully separated, and of these, five major cannabis ingredients were identified. These are cannabinol, cannabidiol, cannabichromene, trans-delta-8-tetrahydrocannabinol, and delta-9-tetrahydrocannabinol. The chromogenic reagent used was the spray of 0.1 per cent solution of Fast blue salt B in 45 per cent ethanol. 1 table, 12 references. (Author abstract modified)