In response to requests from the forensic community, commercial companies are generating larger, more sensitive, and more discriminating STR multiplexes. These multiplexes are now applied to a wider range of samples including complex multi-person mixtures. In parallel there is an overdue reappraisal of profile interpretation methodology. Aspects of this reappraisal include 1) the need for a quantitative understanding of allele and stutter peak heights and their variability; 2) an interest in reassessing the utility of smaller peaks below the often used analytical threshold; 3) a need to understand not just the occurrence of peak drop-in but also the height distribution of such peaks; and 4) a need to understand the limitations of the multiplex-interpretation strategy pair implemented. (Publisher abstract modified)
Validating Multiplexes for Use in Conjunction With Modern Interpretation Strategies
NCJ Number
250072
Journal
Forensic Science International-Genetics Volume: 20 Dated: January 2016 Pages: 6-19
Date Published
January 2016
Length
14 pages
Annotation
This article presents a full scheme for validation of a new multiplex that is suitable for informing modern interpretation practice, predominantly using GlobalFiler as an example multiplex; however, it is suggested that the aspects investigated here are fundamental to introducing any multiplex in the modern interpretation environment.
Abstract