Validation of a Multiplex PCR Assay for the Forensic Identification of Indian Crocodiles

• NCJ Number: 236198
• Journal: Journal of Forensic Sciences Volume: 56 Issue: 5 Dated: September 2011 Pages: 1241-1244
• Author(s): Poorline Ramakodi Meganathan, M.Sc.; Bhawna Dubey, M.Sc.; Kothakota Naga Jogayya, M.Sc.; Ikramul Haque, Ph.D.
• Date Published: September 2011
• Length: 4 pages
• Sponsoring Agency: India Ministry of Home Affairs, Directorate of Forensic Sciences
• Publication Format: Article
• Publication Type: Report (Study/Research)
• Language: English
• Country: United States of America

Annotation

This study validated a novel multiplex polymerase chain reaction assay, developed at the authors' laboratory for the forensic identification of three Indian crocodiles, Crocodylus palustris, Crocodylus porosus, and Gavialis gangeticus, following the guidelines of Scientific Working Group on DNA Analysis Methods.

Abstract

A dependable and efficient wildlife species identification system is essential for swift dispensation of the justice linking wildlife crimes. Development of molecular techniques is befitting the need of the time. The forensic laboratories often receive highly ill-treated samples for identification purposes, and thus, validation of any novel methodology is necessary for forensic usage. The multiplex PCR was tested for its specificity, reproducibility, sensitivity, and stability. This study also includes the samples treated with various chemical substances and exposed to various environmental regimes. The result of this validation study promises this technique to be an efficient identification tool for Indian crocodiles and therefore is recommended for forensic purposes. (Published Abstract)