NCJ Number
236890
Journal
Journal of Forensic Sciences Volume: 56 Issue: 6 Dated: November 2011 Pages: 1574-1579
Date Published
November 2011
Length
6 pages
Annotation
This article gives an overview of the use of immunochromatographic membranes for the detection of prostate-specific antigen (PSA) has become commonplace in forensic laboratories.
Abstract
Use of immunochromatographic membranes for the detection of prostate-specific antigen (PSA) has become commonplace in forensic laboratories. Experiments were designed to test the newly developed Seratec SeraQuaint for accuracy, precision, and consistency in the quantitation of PSA. PSA standards were diluted with buffers and run on the instruments. Values obtained were examined for accuracy (was the correct value obtained?) and precision (were multiple sample values consistent?). To test for variation between instruments, large volumes of diluted PSA standard were run repeatedly on six units, and the values obtained were plotted against the known PSA values to obtain a standard curve for each instrument. Fifty membranes having negative or weak positive results were then run on the six units, and the adjusted values were recorded and compared. Results of these experiments indicate that the instruments are accurate and precise in the quantitation of low levels of PSA. (Published Abstract)